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The 2nd reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water as items (Fig. 9) useful link - circularly polarized luminescence. As attracted Fig - https://urlscan.io/result/3823bc3a-74b6-4d0f-8f09-522e983b4d26/. 9, the response may likewise include electron transfer from H4B to the Fe, IIO2 intermediate
l9 As shown in Fig. 9, this might be since the H4B extreme acts as an electron sink in a later point in the reaction, so as to permit NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.